The nursery has been cleaned by a specialist cleaning company to comply with the requirements of Fife Environmental Health Department and the nursery group's insurers.
The nursery is holding an open afternoon for parents to discuss any concerns before children return to the nursery.
A letter sent to parents said, 'Following the cleaning, extensive microbial sampling and analysis is being undertaken by an independent laboratory to confirm that all harmful bacteria have been destroyed and that the nursery is completely safe for children to return.'
More than 200 clinical waste bags of items including toys, pictures, rugs and cushions were removed and destroyed.
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